The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 01, 2016

Filed:

Mar. 31, 2011
Applicants:

Etsuko Miyamoto, Tokyo, JP;

Toru Tsuji, Tokyo, JP;

Shigeo Fujimori, Tokyo, JP;

Masamichi Ishizaka, Gyeongsangbuk-do, KR;

Inventors:

Etsuko Miyamoto, Tokyo, JP;

Toru Tsuji, Tokyo, JP;

Shigeo Fujimori, Tokyo, JP;

Masamichi Ishizaka, Gyeongsangbuk-do, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 15/64 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1055 (2013.01); C12N 15/1062 (2013.01); C12N 15/1096 (2013.01); C12N 15/64 (2013.01); G01N 33/5308 (2013.01);
Abstract

A method for analyzing biomolecular interactions, which method can be carried out without performing affinity selection using an immobilized bait, is provided. mRNA portions of assignment molecules that interacted with each other are linked together by annealing via a DNA linker for linking mRNA portions of assignment molecules together, the DNA linker comprising, at the 5'-end, an mRNA-complementary region complementary to a sequence at the 5′-end of each mRNA portion, and, at the 3′-end, a self-complementary region complementary between molecules of the DNA linker, the DNA linker being phosphorylated at the 5′-end.


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