The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 01, 2016

Filed:

Jan. 27, 2010
Applicants:

Sumio Ohtsuki, Miyagi, JP;

Junichi Kamiie, Kanagawa, JP;

Tetsuya Terasaki, Miyagi, JP;

Inventors:

Sumio Ohtsuki, Miyagi, JP;

Junichi Kamiie, Kanagawa, JP;

Tetsuya Terasaki, Miyagi, JP;

Assignee:

Tohoku University, Miyagi, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 (2006.01); A61K 38/04 (2006.01); C07K 5/00 (2006.01); C07K 7/00 (2006.01); C07K 16/00 (2006.01); C07K 17/00 (2006.01); C07K 7/06 (2006.01); C07K 7/08 (2006.01); G01N 33/68 (2006.01); H01J 49/00 (2006.01);
U.S. Cl.
CPC ...
C07K 7/06 (2013.01); C07K 7/08 (2013.01); G01N 33/6848 (2013.01); H01J 49/00 (2013.01);
Abstract

Disclosed is an evaluation peptide for evaluating the efficiency of a pretreatment in the quantification of a protein using a mass spectrometer, having high reliability and high general versatility. Also disclosed is an artificial standard protein comprising the evaluation peptide. Further disclosed is a method for quantifying a protein utilizing the artificial standard protein. Specifically disclosed is a method for selecting a peptide which consists of an amino acid sequence not agreeing with that in a naturally occurring protein and a variant thereof and capable of being detected by mass spectrometry and which has an amino acid that can be recognized by a protein-digesting enzyme, and using the peptide as an evaluation peptide for use in the quantification of a protein by a mass spectrometer.


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