The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 25, 2016

Filed:

Oct. 21, 2010
Applicants:

Alexander Vainstein, Rechovot, IL;

Amir Zuker, Nes Ziona, IL;

Inventors:

Alexander Vainstein, Rechovot, IL;

Amir Zuker, Nes Ziona, IL;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8279 (2013.01);
Abstract

A method of generating genotypic variation in a genome of a plant is disclosed. The method comprising introducing into a gamete or a gamete producing tissue of the plant at least one viral expression vector encoding at least one chimeric nuclease which comprises a DNA binding domain, a nuclease and a localization signal to a DNA-containing organelle, wherein the DNA binding domain mediates specific targeting of the nuclease to the genome of the plant, wherein the introducing is performed such that the gamete or gamete producing tissue expresses the chimeric nuclease but not all plant tissues express the chimeric nuclease, thereby generating genotypic variation in the genome of the plant.


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