The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 25, 2016
Filed:
Jan. 09, 2014
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Thang Pham, Mountain View, CA (US);
Arunashree Bhamidipati, Palo Alto, CA (US);
Kevin Travers, Menlo Park, CA (US);
Eric Olivares, Union City, CA (US);
Tyson A. Clark, Menlo Park, CA (US);
Jonas Korlach, Newark, CA (US);
Pacific Biosciences of California, Inc., Menlo Park, CA (US);
Abstract
Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Hook oligonucleotides are used to capture polymerase nucleic acid complexes where the nucleic acids comprise circular nucleic acids having a double stranded central region with hairpin regions on each end. Methods for loading such complexes onto substrates and for single molecule sequencing of such complexes are also provided.