The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 11, 2016

Filed:

Dec. 20, 2013
Applicants:

Universite DE Montreal, Montreal, Quebec, CA;

Mount Sinai Hospital, Toronto, Ontario, CA;

Inventors:

Hugo Lavoie, Montreal, CA;

Malha Sahmi, Ville Lemoyne, CA;

Marc Therrien, Montreal West, CA;

Thanashan Rajakulendran, Scarborough, CA;

Frank Sicheri, Toronto, CA;

Assignees:

MOUNT SINAI HOSPITAL, Toronto, CA;

UNIVERSITÉ DE MONTRÉAL, Montreal, CA;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); C12Q 1/66 (2006.01); C07K 14/47 (2006.01); C12N 9/12 (2006.01); C12Q 1/48 (2006.01); G01N 33/58 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/66 (2013.01); C07K 14/47 (2013.01); C12N 9/12 (2013.01); C12Q 1/485 (2013.01); G01N 33/581 (2013.01); A61K 38/00 (2013.01); G01N 2333/9121 (2013.01); G01N 2333/91205 (2013.01); G01N 2500/02 (2013.01);
Abstract

Disclosed herein are biosensors useful for detecting the dimerization of RAF and/or KSR polypeptides. These biosensors comprise fusion proteins comprising RAF and/or KSR proteins fused to bioluminescent or fluorescent proteins. Also disclosed are methods of using the biosensors to detect and measure the dimerization of RAF/RAF and RAF/KSR polypeptides by resonance energy transfer such as BRET or FRET, for example to screen for inhibitors of dimerization.


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