The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2016

Filed:

Mar. 29, 2011
Applicants:

Huan-chang Lin, Taipei, TW;

Hsin-hung Lin, Taipei, TW;

Cai-yu Kao, Taipei, TW;

Alice L Yu, La Jolla, CA (US);

Wen-ping Peng, Taipei, TW;

Chung-hsuan Chen, Taipei, TW;

Inventors:

Huan-Chang Lin, Taipei, TW;

Hsin-Hung Lin, Taipei, TW;

Cai-Yu Kao, Taipei, TW;

Alice L Yu, La Jolla, CA (US);

Wen-Ping Peng, Taipei, TW;

Chung-Hsuan Chen, Taipei, TW;

Assignee:

Academia Sinica, Taipei, TW;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 45/06 (2006.01); C12Q 1/70 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5091 (2013.01); G01N 33/5008 (2013.01); G01N 33/569 (2013.01); G01N 33/6848 (2013.01);
Abstract

Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided.


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