The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2016

Filed:

Mar. 04, 2011
Applicants:

Chwee Teck Lim, Singapore, SG;

Jongyoon Han, Cambridge, MA (US);

Han Wei Hou, Singapore, SG;

Ali Asgar Bhagat, Singapore, SG;

Krystyn J. Van Vliet, Cambridge, MA (US);

Wong Cheng Lee, Singapore, SG;

Inventors:

Chwee Teck Lim, Singapore, SG;

Jongyoon Han, Cambridge, MA (US);

Han Wei Hou, Singapore, SG;

Ali Asgar Bhagat, Singapore, SG;

Krystyn J. Van Vliet, Cambridge, MA (US);

Wong Cheng Lee, Singapore, SG;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/574 (2006.01); C12Q 1/04 (2006.01); B01L 3/00 (2006.01); C12Q 1/02 (2006.01); G01N 15/14 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/04 (2013.01); B01L 3/50273 (2013.01); B01L 3/502761 (2013.01); C12Q 1/025 (2013.01); B01L 2200/0636 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/086 (2013.01); G01N 2015/149 (2013.01); G01N 2015/1415 (2013.01);
Abstract

A method of detecting one or more diseased blood cells in a blood sample includes introducing a blood sample into at least one inlet of a microfluidic device comprising one or more linear channels wherein each channel has a length and a cross-section of a height and a width defining an aspect ratio adapted to isolate diseased blood cells along at least one portion of the cross-section of the channel based on reduced deformability of diseased blood cells as compared to non-diseased blood cells, wherein diseased blood cells flow along a first portion of the channel to a first outlet and non-diseased blood cells flow along a second portion of the channel to a second outlet. The one or more channels can be adapted to isolate cells along portions of the cross-section of the channel based on cell size. In some embodiments, the one or more channels can be spiral channels.


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