The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 04, 2016

Filed:

Jul. 16, 2013
Applicant:

Kyoto University, Kyoto, JP;

Inventors:

Hirohide Saito, Kyoto, JP;

Kei Endo, Kyoto, JP;

Tan Inoue, Kyoto, JP;

Assignee:

Kyoto University, Kyoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12N 15/63 (2006.01); C12N 15/67 (2006.01); C07K 14/32 (2006.01);
U.S. Cl.
CPC ...
C12N 15/635 (2013.01); C07K 14/32 (2013.01); C12N 15/67 (2013.01);
Abstract

A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5'UTR regulation structure comprising: (1) a cap structure at the 5′ terminus, (2) a spacer positioned on the 3′ side of the cap structure, and (3) one or more RNA motifs positioned on the 3′ side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3′ side of the 5′UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.


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