The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 27, 2016
Filed:
Feb. 01, 2011
Rinji Akada, Ube, JP;
Hisashi Hoshida, Ube, JP;
Babiker Mohamed Ahmed Abdel-banat, Ube, JP;
Jun Asakawa, Ube, JP;
Rinji Akada, Ube, JP;
Hisashi Hoshida, Ube, JP;
Babiker Mohamed Ahmed Abdel-Banat, Ube, JP;
Jun Asakawa, Ube, JP;
Yamaguchi University, Yamaguchi, JP;
Abstract
An object to be solved by the present invention is to provide, for example, a method for producing atransformant by a method for conveniently and efficiently connecting the ends of DNA fragments without using specific restriction enzymes or their recognition sequences, and a method for producing a useful substance using the transformant. The present inventors have found, as means to solve the object, a method comprising introducing two or more linear double-stranded DNA fragments free from aautonomously replicating sequence intoand selecting a transformant comprising a desired DNA ligation product with marker gene expression by the desired DNA ligation product as an index, or comprising introducing a linear double-stranded DNA fragment comprising aautonomously replicating sequence, alone or in combination with one or more linear double-stranded DNA fragment(s) different therefrom intoand selecting a transformant comprising a desired circular DNA ligation product with marker gene expression by the desired circular DNA ligation product as an index.