The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 27, 2016
Filed:
Dec. 21, 2010
Tuomas Huovinen, Turku, FI;
Urpo Lamminmäki, Vanhalinna, FI;
Eeva-christine Brockmann, Turku, FI;
Markus Vehniäinen, Littonen, FI;
Tuomas Huovinen, Turku, FI;
Urpo Lamminmäki, Vanhalinna, FI;
Eeva-Christine Brockmann, Turku, FI;
Markus Vehniäinen, Littonen, FI;
Turun Yliopisto, , FI;
Abstract
The present invention provides a mutagenesis method wherein a nucleic acid molecule is mutagenized with at least one mutagenesis primer in a primer extension reaction and subsequently amplified by rolling circle amplification (RCA). The method involves the step of rendering the template strand unfavorable for RCA. The method involves steps leading to selective amplification of only the mutated strand by a strand-displacing DNA polymerase. Multiple copies of the mutated plasmids are generated during multiple-primed RCA and the resulting DNA is transformed for use. The method is suitable for mutating both single-stranded and double-stranded DNA. The present invention also provides a kit for use in the mutagenesis method.