The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 20, 2016
Filed:
Feb. 22, 2012
Chol Steven Yun, Tucson, AZ (US);
Brian Daniel Kelly, Tucson, AZ (US);
Julia Ashworth-sharpe, Tucson, AZ (US);
Christopher A. Bieniarz, Tucson, AZ (US);
Pascal Bamford, Dublin, CA (US);
Adrian E. Murillo, Tucson, AZ (US);
Chol Steven Yun, Tucson, AZ (US);
Brian Daniel Kelly, Tucson, AZ (US);
Julia Ashworth-Sharpe, Tucson, AZ (US);
Christopher A. Bieniarz, Tucson, AZ (US);
Pascal Bamford, Dublin, CA (US);
Adrian E. Murillo, Tucson, AZ (US);
Ventana Medical Systems, Inc., Tucson, AZ (US);
Abstract
Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.