The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2016

Filed:

Jan. 30, 2012
Applicants:

Sergey V. Voronov, Sharon, MA (US);

Jork Nolling, Hopedale, MA (US);

Inventors:

Sergey V. Voronov, Sharon, MA (US);

Jork Nolling, Hopedale, MA (US);

Assignee:

QIAGEN MANSFIELD, INC., Mansfield, MA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); G01N 27/453 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44743 (2013.01); C12Q 1/686 (2013.01);
Abstract

Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.


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