The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2016
Filed:
Mar. 13, 2014
Butamax Advanced Biofuels Llc, Wilmington, DE (US);
Ritu Bhalla, Tirmulgherry, IN;
Gopal K Chotani, Palo Alto, CA (US);
Michael Dauner, Wilmington, DE (US);
Mark J Nelson, Newark, DE (US);
Daniel P O'Keefe, Ridley Park, PA (US);
Caroline M Peres, Palo Alto, CA (US);
Jahnavi Chandra Prasad, Wilmington, DE (US);
Jean-Francois Tomb, Wilmington, DE (US);
Butamax Advanced Biofuels LLC, Wilmington, DE (US);
Abstract
Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased Kfor NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.