The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2016
Filed:
Mar. 24, 2015
Applicant:
Auxocell Laboratories, Inc., Cambridge, MA (US);
Inventor:
Rouzbeh R. Taghizadeh, Cambridge, MA (US);
Assignee:
Auxocell Laboratories, Inc., Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 5/0775 (2010.01); A61K 35/51 (2015.01); C12N 5/00 (2006.01); C12N 5/07 (2010.01); A61L 27/38 (2006.01); C12N 5/073 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0662 (2013.01); A61K 35/51 (2013.01); A61L 27/3834 (2013.01); C12N 5/00 (2013.01); C12N 5/06 (2013.01); C12N 5/0605 (2013.01); C12N 2500/84 (2013.01); C12N 2502/025 (2013.01);
Abstract
A method of culturing mesenchymal stem cells is provided. The mesenchymal stem cells are cultured in a medium that includes a cell-depleted Wharton's Jelly filtrate. The cell-depleted Wharton's Jelly filtrate is prepared by mincing umbilical cord tissue that includes Wharton's Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Wharton's Jelly stem cells from the filtrate to generate the cell-depleted Wharton's Jelly filtrate.