The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2016
Filed:
Feb. 23, 2007
In-young Song, Seoul, KR;
Hun-taek Kim, Seoul, KR;
Jong-wan Kim, Seoul, KR;
Yong-kook Kim, Youngin-si, KR;
Jong-il Ryu, Yangju-si, KR;
Dae-kee Kim, Seoul, KR;
In-Young Song, Seoul, KR;
Hun-Taek Kim, Seoul, KR;
Jong-Wan Kim, Seoul, KR;
Yong-Kook Kim, Youngin-si, KR;
Jong-Il Ryu, Yangju-si, KR;
Dae-Kee Kim, Seoul, KR;
SK CHEMICALS CO., LTD., Gyeonggi-Do, KR;
Abstract
Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography.