The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 13, 2016

Filed:

Jan. 12, 2010
Applicant:

Masahiko Hase, Tokyo, JP;

Inventor:

Masahiko Hase, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61L 27/38 (2006.01); C12N 5/00 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
A61L 27/3895 (2013.01); C12N 5/0062 (2013.01); A61K 35/12 (2013.01); C12N 2525/00 (2013.01); C12N 2535/10 (2013.01);
Abstract

This invention provides a method for obtaining biological tissue having a three-dimensional structure of interest. The method of the invention comprises: adding a cell-containing culture solution to a culture vessel having an inner bottom surface, which is non-cell adhesive and comprises a concave-convex pattern provided thereon; performing cell culture under conditions in which intercellular adhesion takes place while centrifugal or magnetic force toward the inner bottom surface is applied to the cells that were added to the culture vessel to form tissue via intercellular adhesion; and detaching and collecting the resulting tissue from the inner bottom surface at the end in order to obtain tissue having a three-dimensional configuration using a concave-convex pattern as a template.


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