The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 06, 2016
Filed:
Jul. 17, 2014
Applicant:
Academia Sinica, Taipei, TW;
Inventors:
Assignee:
ACADEMIA SINICA, Taipei, TW;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/58 (2006.01); C12N 9/52 (2006.01); C07K 14/39 (2006.01); C12N 15/52 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/52 (2013.01); C07K 14/39 (2013.01); C12N 15/52 (2013.01); C12P 21/06 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/21 (2013.01); C07K 2319/22 (2013.01); C07K 2319/50 (2013.01); C12Y 304/21 (2013.01);
Abstract
A fusion gene encodingWR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain ofWR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain ofWR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain ofWR-220 keratinase, and use of theWR-220 keratinase.