The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 06, 2016

Filed:

Apr. 20, 2012
Applicants:

Yoshihiro Yoshikawa, Osaka, JP;

Naomi Morikawa, Osaka, JP;

Ryo Tomii, Osaka, JP;

Inventors:

Yoshihiro Yoshikawa, Osaka, JP;

Naomi Morikawa, Osaka, JP;

Ryo Tomii, Osaka, JP;

Assignee:

Nipro Corporation, Osaka, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0656 (2013.01); C12M 23/14 (2013.01); C12M 33/00 (2013.01); C12N 2509/10 (2013.01);
Abstract

Provided is a means suitable for mass culture of adherent cells and which allows easy and safe collection of the cultured adherent cells. A cell culture kit has a culture bag having flexibility, such a level of hydrophilicity that the culture of adherent cells can be achieved, and permeability into a bag shape and which is filled with a culture medium; a dissociation solution bag filled with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof, and a solution sending circuit or a derivative thereof, and a solution sending circuit which connects the culture bag and the dissociation solution bag to each other and can send the dissociation solution from the dissociation solution bag to the culture bag.


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