The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 30, 2016
Filed:
Feb. 25, 2011
Sharon Gerecht, Baltimore, MD (US);
Donny Hanjaya-putra, Baltimore, MD (US);
Elaine Tuong Vo, Baltimore, MD (US);
Maureen Wanjare, Baltimore, MD (US);
Sharon Gerecht, Baltimore, MD (US);
Donny Hanjaya-Putra, Baltimore, MD (US);
Elaine Tuong Vo, Baltimore, MD (US);
Maureen Wanjare, Baltimore, MD (US);
The Johns Hopkins University, Baltimore, MD (US);
Abstract
This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into smooth muscle-like cells (SMLCs) in vitro, comprising a) plating a single-cell suspension of PSCs that are smaller than 50 μm at a seeding concentration of about 5×10cells/cm-about 1×10cells/cmonto a suitable surface, and culturing the cells under conditions which prevent the PSCs from aggregating and induce differentiation of the PSCs into vasculogenic progenitor cells; b) harvesting the cultured cells of step a) and separating them into a single cell suspension of cells that are smaller than 50 μm; and c) plating the single cell suspension of step b) at a seeding concentration of about 1×10cells/cm-about 5×104 cells/cmon a suitable surface, and culturing the cells in a differentiation medium that is supplemented with platelet-derived growth factor BB (PDGF-BB) and transforming growth factor-beta 1 (TGF β1), for a sufficient period of time to allow the vasculogenic progenitor cells to mature into SMLCs. In one embodiment of the invention, the cells from step c) are further subjected to a shear force of at least 1 dyne/cmfor a time period sufficient to enhance differentiation, maturation and/or functionality of the cells.