The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2016

Filed:

Feb. 05, 2009
Applicants:

Hideki Taniguchi, Yokohama, JP;

Yun-wen Zheng, Yokohama, JP;

Go Tazaki, Tsukuba, JP;

Tomoko Kosaka, Tsukuba, JP;

Hitoshi Tsuruta, Ibaraki, JP;

Motohiro Fukuda, Tsukuba, JP;

Inventors:

Hideki Taniguchi, Yokohama, JP;

Yun-Wen Zheng, Yokohama, JP;

Go Tazaki, Tsukuba, JP;

Tomoko Kosaka, Tsukuba, JP;

Hitoshi Tsuruta, Ibaraki, JP;

Motohiro Fukuda, Tsukuba, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12M 23/22 (2013.01); C12M 23/12 (2013.01); G01N 33/5073 (2013.01);
Abstract

Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.


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