The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2016

Filed:

Mar. 08, 2013
Applicant:

Biomèrieux Inc., Durham, NC (US);

Inventors:

Weihua Sonya Wolters, Raleigh, NC (US);

Stanley Michael Philipak, Augusta, MO (US);

Assignee:

bioMérieux, Inc., Durham, NC (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B65B 1/04 (2006.01); B01L 3/00 (2006.01); C12M 1/24 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
B65B 1/04 (2013.01); B01L 3/5082 (2013.01); B01L 3/50825 (2013.01); C12M 23/08 (2013.01); C12M 23/20 (2013.01); C12M 41/26 (2013.01); C12M 41/34 (2013.01); G01N 33/5005 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/141 (2013.01); B01L 2300/0854 (2013.01); B01L 2300/16 (2013.01);
Abstract

Methods of fabricating a culture container include molding a single monolithic layer of polymeric material into a container body having a container shape; introducing a colorimetric sensor material and growth media into the container body; introducing a gas mixture into the container body to define a headspace gas in an upper portion of the container body; attaching a stopper to the neck of the container body with the sensor material; sealing the container body with the stopper closed to define a sealed container with the growth media and the headspace gas enclosed therein; then sterilizing the sealed container; then applying a gas barrier coating to an exterior of the sterilized container body so that the sealed container has an oxygen transmission rate (cubic centimeter/container/day/atm) that is between about 0.001 to about 0.01 to thereby define a culture container that is ready-to-use and shelf stable without autoclaving.


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