The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 23, 2016

Filed:

Jun. 28, 2013
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Maxim Brevnov, Union City, CA (US);

Hemant Pawar, Ann Arbor, MI (US);

Manohar Furtado, San Ramon, CA (US);

Jaiprakash Shewale, Santa Clara, CA (US);

Assignee:

LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C12Q 1/68 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1013 (2013.01); C12N 15/1006 (2013.01); C12Q 1/6806 (2013.01);
Abstract

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.


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