The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 23, 2016
Filed:
Dec. 20, 2013
Applicant:
Thermo Fisher Scientific Baltics Uab, Vilinius, LT;
Inventors:
Remigijus Skirgaila, Vilnius, LT;
Tadas Povilaitis, Vilnius, LT;
Assignee:
Thermo Fisher Scientific Baltics UAB, Vilnius, LT;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1252 (2013.01); C12P 19/34 (2013.01); C12Y 207/07007 (2013.01); Y02P 20/52 (2015.11);
Abstract
Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.