The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 16, 2016
Filed:
Jan. 10, 2002
Hans Söderlund, Espoo, FI;
Kari Kataja, Espoo, FI;
Marja Paloheimo, Vantaa, FI;
Marja Ilmen, Helsinki, FI;
Kristiina Takkinen, Espoo, FI;
Hans Söderlund, Espoo, FI;
Kari Kataja, Espoo, FI;
Marja Paloheimo, Vantaa, FI;
Marja Ilmen, Helsinki, FI;
Kristiina Takkinen, Espoo, FI;
VALTION TEKNILLINEN TUTKIMUSKESKUS, Espoo, FI;
Abstract
The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.