The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 16, 2016

Filed:

Aug. 24, 2012
Applicants:

Shinichi Mizuno, Kurume, JP;

Hidetoshi Ozawa, Kurume, JP;

Koji Nagafuji, Kasuga, JP;

Takashi Okamura, Fukuoka, JP;

Inventors:

Shinichi Mizuno, Kurume, JP;

Hidetoshi Ozawa, Kurume, JP;

Koji Nagafuji, Kasuga, JP;

Takashi Okamura, Fukuoka, JP;

Assignee:

KURUME UNIVERSITY, Fukuoka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01); C12N 15/64 (2006.01); C12N 9/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C07H 21/04 (2013.01); C12N 15/10 (2013.01); C12N 15/64 (2013.01); C12Q 1/6806 (2013.01); C12N 9/93 (2013.01); C12N 15/1003 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6809 (2013.01); C12Q 1/6883 (2013.01); C12Q 2521/301 (2013.01); C12Q 2525/307 (2013.01);
Abstract

The present invention provides a method for producing a circular DNA molecule having a specific structure that enables to distinguish circular DNA formed from a single DNA molecule (single-molecule circular DNA), from circular DNA formed from multiple DNA molecules (multiple-molecule circular DNA) and also from single-molecule circular DNA derived from the circular DNA formed from multiple DNA molecules. According to the present invention, only single-molecule circular DNA that is not derived from multiple-molecule circular DNA can be selected in the production of circular DNA.


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