The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 2016
Filed:
May. 03, 2011
Applicants:
Lei Han, Carmel, IN (US);
Nigel Mouncey, Indianapolis, IN (US);
Inventors:
Lei Han, Carmel, IN (US);
Nigel Mouncey, Indianapolis, IN (US);
Assignee:
Dow AgroSciences LLC, Indianapolis, IN (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/75 (2006.01); C12N 15/10 (2006.01); C12P 19/62 (2006.01); C12P 21/00 (2006.01); C12N 15/52 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1037 (2013.01); C12N 15/52 (2013.01); C12N 15/905 (2013.01); C12P 19/62 (2013.01); C12P 21/00 (2013.01);
Abstract
This invention includes a process for the identification and validation of a neutral polynucleotide integration site within thegenome. In addition, the invention includes the use of the neutral site and methods for the integration of a polynucleotide containing a gene expression cassette, which is stably maintained and expressed over subsequent generations. The invention includes neutral integration sites that can be disrupted without negatively impacting spinosyn production, growth or other desired metabolic characteristics.