The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 2016
Filed:
Dec. 15, 2011
Michel Revel, Rehovot, IL;
Judith Chebath, Rehovot, IL;
Guy Slutsky, Holon, IL;
Alon Levy, Ramat-HaSharon, IL;
Michal Izrael, Rehovot, IL;
Arik Hasson, Kiryat-Ono, IL;
Kfir Molakandov, Yahud, IL;
Rosalia Kaufman, Rehovot, IL;
Michel Revel, Rehovot, IL;
Judith Chebath, Rehovot, IL;
Guy Slutsky, Holon, IL;
Alon Levy, Ramat-HaSharon, IL;
Michal Izrael, Rehovot, IL;
Arik Hasson, Kiryat-Ono, IL;
Kfir Molakandov, Yahud, IL;
Rosalia Kaufman, Rehovot, IL;
Kadimastem Ltd., Nes-Ziona, IL;
Abstract
A method of generating islet cells from pluripotent stem cells is disclosed. The method comprises: (a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and (b) culturing the endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and (c) culturing the further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells from pluripotent stem cells. Further methods of generating islet cells are also disclosed, isolated cell populations comprising same and uses thereof.