The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 02, 2016

Filed:

Jun. 20, 2012
Applicants:

Yoshikazu Yonemitsu, Fukuoka, JP;

Yui Harada, Fukuoka, JP;

Satoru Saito, Fukuoka, JP;

Yuichiro Yazaki, Minato-ku, JP;

Masato Okamoto, Minato-ku, JP;

Takefumi Ishidao, Minato-ku, JP;

Inventors:

Yoshikazu Yonemitsu, Fukuoka, JP;

Yui Harada, Fukuoka, JP;

Satoru Saito, Fukuoka, JP;

Yuichiro Yazaki, Minato-ku, JP;

Masato Okamoto, Minato-ku, JP;

Takefumi Ishidao, Minato-ku, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 35/28 (2015.01); A61K 38/00 (2006.01); C12N 5/0783 (2010.01); A61K 35/17 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0646 (2013.01); A61K 35/17 (2013.01); C12N 2501/2302 (2013.01);
Abstract

A technique is needed which can amplify NK cells in vitro and prepare optimum number of NK cells for the adoptive immunotherapy. A method for amplifying NK cells is provided which comprises steps of: preparing cell population which is comprised of NK cells, removing T cells from the cell population which is comprised of NK cells, and, after removal of T cells, cultivating the remaining cells in a medium supplemented with 2500 to 2831 IU/mL of IL-2. The method for amplifying NK cells of the present invention may comprise a step of removing hematopoietic progenitor cells from the cell population. The present invention provides a pharmaceutical composition for adoptive immunotherapy, comprising NK cells which are prepared by the amplifying method of the present invention.


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