The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 2016
Filed:
Dec. 02, 2011
Tatsutoshi Nakahata, Kyoto, JP;
Kohichiro Tsuji, Tokyo, JP;
Feng MA, Tokyo, JP;
Hirohisa Saito, Tokyo, JP;
Kenji Matsumoto, Tokyo, JP;
Tatsutoshi Nakahata, Kyoto, JP;
Kohichiro Tsuji, Tokyo, JP;
Feng Ma, Tokyo, JP;
Hirohisa Saito, Tokyo, JP;
Kenji Matsumoto, Tokyo, JP;
KYOTO UNIVERSITY, Kyoto-shi, Kyoto, JP;
Abstract
The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.