The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 2016
Filed:
Mar. 24, 2010
Shinichi Yoshida, Hyogo, JP;
Dai Murata, Hyogo, JP;
Masayuki Takano, Hyogo, JP;
Junya Akagi, Hyogo, JP;
Keita Iguchi, Hyogo, JP;
Yoshiyuki Nakano, Hyogo, JP;
Shinichi Yoshida, Hyogo, JP;
Dai Murata, Hyogo, JP;
Masayuki Takano, Hyogo, JP;
Junya Akagi, Hyogo, JP;
Keita Iguchi, Hyogo, JP;
Yoshiyuki Nakano, Hyogo, JP;
KANEKA CORPORATION, Osaka-shi, Osaka, JP;
Abstract
An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.