The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 26, 2016

Filed:

Sep. 25, 2013
Applicants:

Arati Sridharan, Chandler, AZ (US);

Jitendran Muthuswamy, Chandler, AZ (US);

Inventors:

Arati Sridharan, Chandler, AZ (US);

Jitendran Muthuswamy, Chandler, AZ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 13/00 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); C12N 15/111 (2013.01); C12N 2310/14 (2013.01); C12N 2320/32 (2013.01);
Abstract

Described herein are methods and compositions for high efficiency transfection of siRNA into a cell population. Such methods and compositions utilize a low voltage pre-conditioning pulse to modulate the efficiency of siRNA transfection. In some embodiments, the methods and compositions permit spatial and temporal control of siRNA transfection efficiency within a population of cells. The disclosed methods and compositions, in some embodiments, are amenable to high throughput applications such as siRNA library-based phenotypic screening.


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