The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 26, 2016
Filed:
Feb. 18, 2010
Hans-willi Krell, Penzberg, DE;
Alexander Lifke, Penzberg, DE;
Valeria Lifke, Penzberg, DE;
Kairat Madin, Penzberg, DE;
Christian Weilke, Penzberg, DE;
Hans-Willi Krell, Penzberg, DE;
Alexander Lifke, Penzberg, DE;
Valeria Lifke, Penzberg, DE;
Kairat Madin, Penzberg, DE;
Christian Weilke, Penzberg, DE;
Hoffmann-La Roche Inc., Little Falls, NJ (US);
Abstract
The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps: —performing a first polymerase chain reaction with three to six 5'-primer and one 3′-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5′-primer and one 3′-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.