The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2016
Filed:
Apr. 25, 2008
Thomas M. Ramseier, Carmel, IN (US);
Russell J. Coleman, San Diego, CA (US);
Jane C. Schneider, San Diego, CA (US);
Charles D. Hershberger, Fremont, CA (US);
Diane M. Retallack, Poway, CA (US);
Charles H. Squires, Poway, CA (US);
Thomas M. Ramseier, Carmel, IN (US);
Russell J. Coleman, San Diego, CA (US);
Jane C. Schneider, San Diego, CA (US);
Charles D. Hershberger, Fremont, CA (US);
Diane M. Retallack, Poway, CA (US);
Charles H. Squires, Poway, CA (US);
PFENEX INC., San Diego, CA (US);
Abstract
The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.