The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2016
Filed:
Nov. 29, 2006
Alexander Seitz, Vienna, AT;
Alexander Seitz, Vienna, AT;
LEXOGEN GMBH, Vienna, AT;
Abstract
The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3' end of the transcribed cDNA with a polynucleotide tail after the 3′ end, c) amplification of the cDNA using a pair of primers, wherein a first 3′ primer is specific for the 5′ end of the cDNA and a second 5′ primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3′polynucleotide tail of the cDNA.