The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 19, 2016

Filed:

Jun. 30, 2015
Applicant:

Sonogene Llc, Glen Ellyn, IL (US);

Inventors:

Eric Coles, Glen Ellyn, IL (US);

Michael Davidson, Highland Park, IL (US);

Assignee:

SONOGENE LLC, Glen Ellyn, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 49/22 (2006.01); A61K 31/713 (2006.01); A61K 31/7105 (2006.01); C12N 15/85 (2006.01); A61M 37/00 (2006.01); A61K 31/7088 (2006.01); A61K 45/06 (2006.01); A61K 41/00 (2006.01); A61K 48/00 (2006.01); C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
A61K 49/223 (2013.01); A61K 31/7088 (2013.01); A61K 31/713 (2013.01); A61K 31/7105 (2013.01); A61K 41/0028 (2013.01); A61K 45/06 (2013.01); A61K 48/00 (2013.01); A61M 37/0092 (2013.01); C12N 15/00 (2013.01); C12N 15/85 (2013.01);
Abstract

The present invention provides methods for transfecting cells (e.g., liver cells) to express ABCA1 and increase HDL in the blood. The compositions described herein comprise a pharmaceutically acceptable aqueous carrier containing sonochemically-active microspheres together with a plasmid DNA construct encoding an active form of ABCA1 and at least one promoter for the expression thereof. Preferably, the sonochemically-active microspheres comprise, consist essentially of, or consist of gas bubbles (e.g., a fluorocarbon gas, such as octafluoropropane) encapsulated within protein-containing or lipid-containing shells (e.g., human serum albumin shells). The microspheres are disruptable by exposure to ultrasonic acoustic energy to release the encapsulated gas.


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