The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 2016
Filed:
Aug. 24, 2012
Dean W. Gabriel, Alachua, FL (US);
Giovana J. Perazzo-ratto, Alachua, FL (US);
Yingnan Jiang, Alachua, FL (US);
Dean W. Gabriel, Alachua, FL (US);
Giovana J. Perazzo-Ratto, Alachua, FL (US);
Yingnan Jiang, Alachua, FL (US);
INTEGRATED PLANT GENETICS, INC., Gainesville, FL (US);
Abstract
The invention relates to methods and compositions for genetic transformation of both juvenile and mature citrus. In some embodiments, the invention provides methods and compositions for genetic transformation of citrus using Rhizobia-mediated DNA delivery, and also methods of enhancing the frequency of genetic transformation of mature citrus by any DNA transfer method, including. Internodal stem sections prepared from epicotyls of citrus seedlings or freshly emerging shoots of mature citrus plants (e.g., first shoots from buds of mature plants following grafting onto rootstock or very young shoots of mature plants) are preconditioned for transformation by inducing callus formation on an artificial medium. All callus and any developing meristematic regions in immediately adjacent tissue are substantially or completely removed and the preconditioned explants are then transformed byor other known methods. Whole plants or shoots to be grafted onto rootstocks are subsequently regenerated from the transformed cells.