The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 12, 2016

Filed:

May. 28, 2014
Applicant:

Genvec, Inc., Gaithersburg, MD (US);

Inventors:

Jason G. D. Gall, Germantown, MD (US);

Douglas E. Brough, Gaithersburg, MD (US);

C. Richter King, New York, NY (US);

Assignee:

GenVec, Inc., Gaithersburg, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/01 (2006.01); C12N 15/861 (2006.01); C12N 15/86 (2006.01); C12N 7/00 (2006.01); C12N 15/63 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C12N 7/00 (2013.01); C12N 15/635 (2013.01); C12N 2710/10343 (2013.01); C12N 2710/10352 (2013.01); C12N 2830/006 (2013.01);
Abstract

The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (b) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.


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