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Abstract

Genes SWA2 and GAM1 from the yeast,, encoding α-amylase and glucoamylase, respectively, were cloned and expressed in. The expression was achieved by integration of the SWA2 and GAM1 genes into the chromosome ofunder operably linked to a strong constitutive promoter of the-glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus, and a xlnD gene coding for β-xylosidase was obtained from the fungus. Co-expression of these genes was also achieved by integration into thechromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in astrain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.