The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 12, 2016

Filed:

Oct. 01, 2010
Applicants:

Troels Kasper Høyer Scheel, Copenhagen NV, DK;

Judith M. Gottwein, Frederiksberg C, DK;

Tanja Bertelsen Jensen, Frederiksberg C, DK;

Jens Bukh, Praestø, DK;

Inventors:

Troels Kasper Høyer Scheel, Copenhagen NV, DK;

Judith M. Gottwein, Frederiksberg C, DK;

Tanja Bertelsen Jensen, Frederiksberg C, DK;

Jens Bukh, Praestø, DK;

Assignees:

Hvidovre Hospital, Hvidovre, DK;

Kobenhavns Universitet, Copenhagen K, DK;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C07K 14/005 (2006.01); C12Q 1/18 (2006.01); G01N 33/569 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C07K 14/005 (2013.01); C12Q 1/18 (2013.01); G01N 33/56983 (2013.01); A61K 39/00 (2013.01); C12N 2770/24222 (2013.01); C12N 2770/24251 (2013.01); G01N 2333/18 (2013.01);
Abstract

The present inventors developed hepatitis C virus recombinants expressing NS5A from genotype 1a, 1b, 2a, 3a, 4a, 5a, 6a or 7a in the context of a genotype 2a backbone. Additional recombinants express NS5A and the structural proteins (Core, E1 and E2), p7 and NS2 from genotype 1a, 1b, 3a, 4a, 5a, 6a or 7a in the genotype 2a backbone. Sequence analysis of the recombinants recovered after viral passage in Huh7.5 cells revealed adaptive mutations in NS5A and/or NS3. The importance of these mutations for improved growth kinetics was shown in reverse genetic studies.


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