Hydrophobic cellulose membrane, method for the production thereof, and use of same in hydrophobic interaction chromatography

Abstract

There is provided a crosslinked cellulose hydrate membrane having a porous double structure which consists of micropores having a diameter in the range from >100 nm to 20 μm and ultrapores which have a diameter of <100 nm and which are not accessible to Blue Dextran having an average molecular weight Mw of 2 000 000, wherein the fraction of the volume of the ultrapores is more than 15% of the entire pore volume accessible to water, and wherein hydrophobic ligands, selected from C-C-alkyl and their derivatives or C-C-aryl and their derivatives or C-C-arylalkyl and their derivatives or —[(CH)—O—]—R, where m is 2 or 3, n is a whole number greater than or equal to 1, and R is —H or —C-C-alkyl, are bonded to the membrane. In addition, methods for producing the membrane, an apparatus for hydrophobic interaction chromatography and comprising the membrane, and the use of the membrane in hydrophobic interaction chromatography are specified.