The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 05, 2016
Filed:
Aug. 10, 2009
Vyvyan Clare Salisbury, Bristol, GB;
Habib Mahmoud Alloush, Bristol, GB;
Margaret Ann Smith, Bristol, GB;
Paul John Innocenzi, Crumlin, GB;
Mark William Ruddock, Crumlin, GB;
Ashley Diane Martin, Crumlin, GB;
Vyvyan Clare Salisbury, Bristol, GB;
Habib Mahmoud Alloush, Bristol, GB;
Margaret Ann Smith, Bristol, GB;
Paul John Innocenzi, Crumlin, GB;
Mark William Ruddock, Crumlin, GB;
Ashley Diane Martin, Crumlin, GB;
RANDOX LABORATORIES LTD., , GB;
UNIVERSITY OF THE WEST OF ENGLAND, Bristol, GB;
Abstract
The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug.