The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 05, 2016
Filed:
Dec. 20, 2013
Applicant:
Agriculture and Agri-food Canada, Guelph, CA;
Inventors:
Francois Eudes, Lethbridge, CA;
Archana Chugh, Lethbridge, CA;
Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8202 (2013.01); C12N 15/8206 (2013.01); C12N 15/8218 (2013.01); C12N 15/8263 (2013.01); C12N 15/87 (2013.01); C07K 2319/10 (2013.01);
Abstract
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.