The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2016
Filed:
Aug. 11, 2010
Arthur H. Reis, Jr., Arlington, MA (US);
Lawrence J. Wangh, Auburndale, MA (US);
Kenneth Pierce, Natick, MA (US);
Arthur H. Reis, Jr., Arlington, MA (US);
Lawrence J. Wangh, Auburndale, MA (US);
Kenneth Pierce, Natick, MA (US);
Brandeis University, Waltham, MA (US);
Abstract
Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of variousspecies and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community strain by detecting the presence of absence of one or more target sequences selected from a list of target genes and sequences that include, but are not limited to: mecA, femA-SA, femA-SE, lukF-PV, lukS-PV, VanA, SCCmec type I, type II, type III, type IV, type V, and type VII. In certain embodiments, asymmetric PCR amplification methods are employed. In other embodiments, probe-target generated signals are detected at least two temperatures to generate a temperature/temperature signal ratio.