The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2016

Filed:

Apr. 11, 2014
Applicant:

Ajinomoto Co., Inc., Tokyo, JP;

Inventors:

Noriko Tsurui, Kanagawa, JP;

Hiroshi Itaya, Kanagawa, JP;

Yoshimi Kikuchi, Kanagawa, JP;

Assignee:

AJINOMOTO CO., INC., Tokyo, JP;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/02 (2006.01); C07K 14/34 (2006.01); C12N 15/77 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 14/34 (2013.01); C12N 15/77 (2013.01); C07K 2319/02 (2013.01); C07K 2319/50 (2013.01);
Abstract

A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium is cultured so that it secretes a heterologous protein, the bacterium having a genetic construct which includes a promoter sequence that functions in the coryneform bacterium, a nucleic acid sequence coding for a signal peptide that functions in the coryneform bacterium, which is ligated downstream from the promoter sequence, and a nucleic acid sequence coding for a fusion protein having an amino acid sequence that includes Gln-Glu-Thr and the heterologous protein, which is ligated downstream from the nucleic acid sequence coding for the signal peptide.


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