The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2016
Filed:
Oct. 31, 2011
Shoen Kume, Kumamoto, JP;
Soichiro Ogaki, Fukuoka, JP;
Nobuaki Shiraki, Kumamoto, JP;
Kazuhiko Kume, Kumamoto, JP;
Shoen Kume, Kumamoto, JP;
Soichiro Ogaki, Fukuoka, JP;
Nobuaki Shiraki, Kumamoto, JP;
Kazuhiko Kume, Kumamoto, JP;
National University Corporation Kumamoto University, Kumamoto-Shi, JP;
LSIP, LLC, Tokyo, JP;
Abstract
An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2'Z,3′E)-6-bromoindirubin-3′-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells.