The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 14, 2016
Filed:
Nov. 22, 2013
Life Technologies Corporation, Carlsbad, CA (US);
Theo Nikiforov, Carlsbad, CA (US);
Daniel Mazur, San Diego, CA (US);
Xinzhan Peng, Carlsbad, CA (US);
Tommie Lloyd Lincecum, Houston, TX (US);
Yuri Belosludtsev, The Woodlands, TX (US);
Howard Reese, Poway, CA (US);
Dmitriy Gremyachinskiy, San Francisco, CA (US);
Roman Rozhkov, Redwood City, CA (US);
John Mauro, Burlingame, CA;
Joseph Beechem, Eugene, OR (US);
Eric Tulsky, Berkeley, CA (US);
Imad Naasani, Manchester, GB;
Kari Haley, Eugene, OR (US);
Joseph Treadway, Eugene, OR (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.