The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 07, 2016

Filed:

Oct. 31, 2006
Applicants:

Yuki Katayama, Tokyo, JP;

Kazuhito Miyauchi, Tokyo, JP;

Shizuyo Takada, Tokyo, JP;

Tomomi Murakami, Tokyo, JP;

Inventors:

Yuki Katayama, Tokyo, JP;

Kazuhito Miyauchi, Tokyo, JP;

Shizuyo Takada, Tokyo, JP;

Tomomi Murakami, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/78 (2006.01); G01N 33/92 (2006.01); C12Q 1/44 (2006.01); C12Q 1/61 (2006.01);
U.S. Cl.
CPC ...
G01N 21/78 (2013.01); C12Q 1/44 (2013.01); C12Q 1/61 (2013.01); G01N 33/92 (2013.01); G01N 2333/918 (2013.01); G01N 2333/91215 (2013.01);
Abstract

The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii),and a kit used for the method.


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