The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 07, 2016
Filed:
Oct. 16, 2012
Bgi Shenzhen Co., Limited, Shenzhen, CN;
Bgi Shenzhen, Shenzhen, CN;
Xuyang Yin, Shenzhen, CN;
Chunlei Zhang, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Xiuqing Zhang, Shenzhen, CN;
Shengpei Chen, Shenzhen, CN;
BGI SHENZHEN CO., LIMITED, Shenzhen, CN;
BGI SHENZHEN, Shenzhen, CN;
Abstract
Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.