The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 07, 2016

Filed:

Jul. 27, 2012
Applicants:

Thomas Tuschl, Brooklyn, NY (US);

Pavol Cekan, Gaithersburg, MD (US);

Neil Renwick, New York, NY (US);

Inventors:

Thomas Tuschl, Brooklyn, NY (US);

Pavol Cekan, Gaithersburg, MD (US);

Neil Renwick, New York, NY (US);

Assignee:

The Rockefeller University, New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 1/30 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6806 (2013.01); C12N 15/111 (2013.01); G01N 1/30 (2013.01); C12N 2310/141 (2013.01); C12N 2320/10 (2013.01); C12Q 2600/178 (2013.01);
Abstract

The invention provides a method for fixing an RNA molecule in a biological sample. The method comprises contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a solution comprising a carbodiimide and a heterocyclic derivative selected from the group consisting of an imidazole, pyrazole, triazole or tetrazole or a combination thereof. A method for differentiating cells in a biological sample is also provided. A method for quantification of RNA retention in a biological sample is also provided. A kit for fixing RNA in a sample is provided as well.


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