The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 31, 2016

Filed:

Oct. 24, 2011
Applicants:

Kenneth J. Livak, San Jose, CA (US);

Jason A. A. West, Pleasanton, CA (US);

Robert C. Jones, Los Altos, CA (US);

Inventors:

Kenneth J. Livak, San Jose, CA (US);

Jason A. A. West, Pleasanton, CA (US);

Robert C. Jones, Los Altos, CA (US);

Assignee:

Fluidigm Corporation, South San Francisco, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/682 (2013.01); C12Q 1/682 (2013.01); C12Q 2525/155 (2013.01); C12Q 2525/307 (2013.01); C12Q 2537/161 (2013.01);
Abstract

Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.


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